水稻籽粒灌浆过程中蛋白质表达特性及其对氮肥运筹的响应 |
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水稻籽粒灌浆过程中蛋白质表达特性及其对氮肥运筹的响应 Expression characteristics of grain protein and its response to Nitrogen application during the grain-filling stage of rice (Oryza Sativa.L) 发布时间：2011-06-01 浏览量：103 收藏数：0 评论数：0 总览 评价 张志兴1， 李忠2， 陈军2， 李兆伟2， 黄锦文2， 陈婷2， 方长旬2， 林文雄2* （ 1、福建农林大学生命科学学院农业生态研究所； 2、福建农林大学生命科学学院农业生态研究所，福州，350002； ）
摘要： 以大穗型水稻为材料，运用双向电泳技术研究了籽粒灌浆不同时段蛋白质的表达变化，共发现32个发生显著表达差异的蛋白质。随后，进一步构建了水稻生育前后期2种不同氮肥比例下（7：3 和6：4）强弱势籽粒灌浆不同时段的蛋白表达图谱，发现强势籽粒响应氮肥调控比弱势籽粒稳定，发生差异表达并得到功能鉴定的蛋白质为4对17。结果表明，籽粒灌浆是个复杂的生态学过程，涉及淀粉合成，能量代谢，激素信号转导，基因表达调节等过程。进一步分析发现，强势粒灌浆具有明显的环境稳定性，而弱势粒灌浆则受环境因素（如氮肥运筹）的强烈调节。对大穗型水稻而言，适当增加生育后期氮肥的施用量，有利于通过增强信号转导，促进相关基因的表达，提高物质调运与能量代谢速率，增强抗逆性，增强弱势籽粒的代谢水平，延长其灌浆时期，提升弱势籽粒活性和灌浆强度。
关键词： 水稻；籽粒灌浆；生态；蛋白组学；氮肥 ZHANG Zhixing1， LI Zhong2， CHEN Jun2， LI Zhaowei2， HUANG Jinwen2， CHEN Ting2， FANG Changxun2， LIN Wenxiong2* （ 1、Institute of Agricultural Ecology, School of Life Sciences, Fujian Agriculture and Forestry University； 2、Institute of Agricultural Ecology, School of Life Sciences, Fujian Agriculture and Forestry University, Fujian, Fuzhou, 350002； ）
Abstract： The 2-D PAGE was employed to separate proteins of grain in the different grain-filling stages of large panicle rice. Then, the different nitrogen application ratios (7:3 and 6:4) were set to clarify the different response mechanisms of superior and inferior grains. 2D maps of grain proteome at different time courses during grain-filling stage were got with 32 differentially expressed proteins were detected by Imagemaster software, while 27 proteins were successfully identified by MS. Then, differential expression patterns proteins in superior and inferior grains at different time courses responding to different N application ratios were further analysis. The results showed 26 differentially expressed proteins with 17 identified in inferior grains, while 9 proteins with differential expressions were detected in superior grains and 4 were successfully identified. Grain-filling is a complicated ecological process, involving starch synthesis, energy metabolism, signal transduction, genes expression and regulation and so on. These molecular mechanisms act together to advance the seed whole development, from germination to vegetative development, productive development, and to the natural senescence. The grain-filling of superior spikelet showed environmental stability, but the grain-filling of inferior spikelet could be regulated by the environment factor (such as Nitrogen application).The increasing N application amounts in grain-filling phases of rice favored in the up-regulation of the genes involved in physiological activities of inferior grain filling, including the signals transduction, gene expression, energy accumulation for the material metabolism, and hormone production etc. in inferior grains of rice, in turn, leading to increased yield.
Keywords： Rice; Grain filling; Ecology; Proteomics; Nitrogen fertilizer
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