大蒜素诱导体外培养的小鼠淋巴细胞（el-4）凋亡的研究 |
|
|
大蒜素诱导体外培养的小鼠淋巴细胞（el-4）凋亡的研究 收藏此文
全部作者 ： 曹志恒 王子仁
第一作者单位 ： 兰州大学生命科学学院
论文摘要 ： objective: the present study was undertaken to evaluate anti-proliferative and pro-apoptotic activities of allicin—a plant derived organics sulfur compounds (osc) in murine t-lymphocytes (el-4) and try to determine its molecular mechanism of inducing apoptosis. methods: trypan blue dye exclusion method was used to determine cells concentration, the apoptotic rate and morphologic alteration of nuclei were measured by hoechst 33258 fluorescent dye. dna spallation was determined by using tunel kit. protein expression of bax, bcl-2, cythrome c, caspase-3, -12 were detected by immunohistochemistry (ihc). fluorescent microscope was used to determine the change of mitchrodria membrane potential (mmp). result: allicin is effective in inhibiting the proliferation of el-4 cells in vitro in concentration-dependent manner, induces the formation of apoptotic bodies, nuclear condendensation, dna spallation, and it even activates the expression of caspase-3, -12, cytochrome c, upregulates the ratio of the heterodimer bax-bcl-2 and induces mmp decrease. conclusions: allicin may induce apioptosis in el-4 cells with a time- and concentration-dependent manner, in which the mitochnodria pathway may play a central role.
关键词 ： allicin, el4 cell, anti-tumor, apoptosis, mmp
发表日期 ： 2008年02月02日
同行评议 ： （暂时没有） 综合评价：
大蒜素诱导体外培养的小鼠淋巴细胞（el-4）凋亡的研究 来自: 免费论文网www.paper800.com
远程下载:《大蒜素诱导体外培养的小鼠淋巴细胞（el-4）凋亡的研究》论文(PDF全文)
|
|
|
|
|
|
|
