molecular cloning and characterization of leebf1 and leebf2 from tomato |
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molecular cloning and characterization of leebf1 and leebf2 from tomato 收藏此文
全部作者 ： 龙皎月 胡宗利 陈绪清 严波 陈国平
第一作者单位 ： 重庆大学生物工程学院
论文摘要 ： two full-length cdnas of leebf1 and leebf2 were cloned from tomato (solanum lycopersicum) by rt-pcr and rapid amplification of cdna ends (race). they were 2866 and 2891 bp, with open reading frame (orf) of 1911 bp and 1995 bp, encoding 637 and 665 amino acids, respectively. putative leebf1 and leebf2 proteins, each containing a well-conserved f-box motif in the amino terminus and 14 tandem leucine-rich repeats (lrrs) in the carboxyl terminus, shared 58.6% of identity with each other. identities with other species ebf proteins within f box motif ranged from 24.4% to 73.2%.
关键词 ： leebf1; leebf2; tomato; ubiquitination
发表日期 ： 2008年02月21日
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